A simple assay for monoamine oxidase using glutathione peroxidase and glutathione reductase.
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概要
- 論文の詳細を見る
A new fluorometric assay for the determination of monoamine oxidase activity that is applicable to any substrates including dopamine and serotonin is described. Hydrogen peroxide formed during the monoamine oxidase reaction was reduced in the presence of glutathione and glutathione peroxidase, and the oxidized glutathione was measured fluorometrically as NADP<SUP>+</SUP> via oxidation of NADPH by glutathione reductase. This method was applied for inhibitor studies using clorgyline and deprenyl.
- 公益社団法人 日本薬理学会の論文
著者
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Nagatsu Toshiharu
Laboratory Of Cell Physiology Department Of Life Chemistry Graduate School At Nagatsuta
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Kawai Satoshi
Gifu College of Pharmacy
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NAKANO Tamotsu
Laboratory of Cell Physiology, Department of Life Chemistry, Graduate School at Nagatsuta, Tokyo Institute of Technology
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