Involvement of Mitochondrial Genes of Babesia gibsoni in Resistance to Diminazene Aceturate

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概要

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• The stability of the characteristics of the diminazene aceturate (DA)-resistant B. gibsoni isolate was initially determined invitro. Part of the DA-resistant B. gibsoni isolate was cultured without DA for 4 weeks, and then newly exposed to 200 ng/ml DA. As a result, this isolate could proliferate the same as the DA-resistant isolate, indicating that the characteristic of DA resistance was stable in the DA-resistant isolate. Additionally, the level of parasitemia in the DA-resistant isolate was comparatively lower than in the wild-type, suggesting that the proliferation potential of the DA-resistant isolate would be lower than that of the wild-type. Subsequently, to investigate the involvement of mitochondrial DNA (mtDNA) in DA resistance in B. gibsoni, the nucleotide sequences and deduced amino acid sequences of mitochondrial genes such as COXI, COXIII, and CYTb genes of the DA-resistant isolate, were compared with those of the wild-type. As a result, these three genes were not altered in the DA-resistant B. gibsoni isolate. Moreover, the transcription levels of COXI, COXIII, and CYTb genes were observed by semi-quantitative RT-PCR. As a result, the gene transcription of those genes in the DA-resistant isolate was not significantly altered. These results indicated that DA did not affect mtDNA directly in DA-resistant B. gibsoni. Thus, it is suggested that mtDNA should not be deeply involved in DA resistance in B. gibsoni.

著者

• Takiguchi Mitsuyoshi

  Laboratory Of Pathobiology Department Of Veterinary Clinical Sciences Graduate School Of Veterinary

• Hwang Shiang-Jyi

  Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate Sch

• SASAKI Noboru

  Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate Sch

• WICKRAMASEKARA RAJAPAKSHAGE

  Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate Sch

• Nakamura Kensuke

  Laboratory Of Veterinary Internal Medicine Department Of Veterinary Clinical Sciences School Of Vete

• Yamasaki Masahiro

  Laboratory Of Internal Medicine Department Of Veterinary Clinical Science Graduate School Of Veterin

• Ohta Hiroshi

  Laboratory For Genomic Reprogramming Center For Developmental Biology Riken

• Murakami Masahiro

  Laboratory Of Marine Biochemistry Graduate School Of Agricultural Life Sciences The University Of To

• LIM Sue

  Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University

• TAMURA Yu

  Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University

• WICKRAMASEKARA RAJAPAKSHAGE

  Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University

• WICKRAMASEKARA RAJAPAKSHAGE

  Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan

• SASAKI Noboru

  Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan

• NAKAMURA Kensuke

  Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan

• OHTA Hiroshi

  Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan

• TAKIGUCHI Mitsuyoshi

  Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan

• HWANG Shiang-Jyi

  Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan

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