副鼻腔癌の電子顕微鏡的観察

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Tissues from 41 patients with paranasal carcinoma (39 maxillary and 2 ethmoidal sinuses) obtained by biopsy were observed under light and electron microscopy. Twenty were squamous cell carcinoma, 9 adenocarcinoma, 3 adenoid cystic carcinoma, 2 mucoepidermoid carcinoma and 1 adenosquamous carcinoma.Tissue for light microscopy was fixed in formalin, embedded in paraffin and stained with hematoxylin and eosin. PAS, PAS-diastase digestion, alcian blue and mucicarmin stains were used as required. For electron microscopy, minced tissue blocks were fixed in 2.5% glutaraldehyde for 1 hour and post-fixed in 1% osmium tetraoxide for 1 hour, dehydrated in graded concentration of ethanol and embedded in epon. Thin sections were doubly stained with uranyl acetate and lead citrate, and examined under a Hitachi HIJ-11DS and JEM 100C electron microscope.From the ultrastructural observation of each tumor type, it was revealed that one poorly differentiated squamous cell carcinoma and two poorly differentiated adenocarcinoma were reevaluated in the six undifferentiated carcinomas diagnosed under light microscopy. One adenosquamous carcinoma and a carcinoid were found in the solid type of adenoid cystic carcinoma diagnosed by routine pathological examination.The grade of differentiation in the squamous cell carcinoma and adenocarcinoma evaluated by light microscopic findings, was reevaluated using electron microscopy. In consequence, the following cytoplasmic and nuclear makers were varied with the differential gradings of the cancer cells: the mode of cell junction, the number and size of cell organelles and nuclear features.Histogenesis of paranasal carcinoma was discussed on the basis of ultrastructural similarity to "surface epithelial", "ductal" and "acinar" cells of normal human maxillary sinus.
耳鼻咽喉科臨床学会の論文

副鼻腔癌の電子顕微鏡的観察

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