百万ボルト電子顕微鏡の生物試料への応用-1-内耳有毛細胞の観察

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The thick sections of the sensory hair cells and nerve endings of the guinea pig cochlea were observed under the high voltage electron microscope (HVEM). The main advantage of HVEM is that sharp images of thicker specimens can be obtained, as the result of the greater penetrating power of high-energy electrons.The thickness between 600nm and 1000nm was the optimum range for observation of the inner ear specimens under 1000kV accelerating voltage. Specimens less than 600nm in thickness often lacked sufficient contrast of image, and specimens over 1000nm in thickness were too thick to be clearly defined. The uranyl acetate alcohol solution and lead acetate aqueous solution were used as stains.The fine structure of the cell organella such as mitochondria, synaptic vesicles, and ERs were often poorly defined due to their increased electron density. However, we concluded that the 1000nm specimens were quite useful for the numerical studies of the nerve endings since the serial thick sections were easily obtained on the grids and the differentiation between the efferent and afferent was possible under HVEM.
耳鼻咽喉科臨床学会の論文