In Vivo Action of Pronase-P:(Inhibitory Action of Serum on Pronase-P)

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About 70% of the patients with chronic sinusitis showed a big release of the symptoms by the administration of Pronase-P. The chronic inflammatory change in mucous membrane of sinus was found to be gradually decreased.Pronase-P activity measured both by casein and L-leucyl. β. naphthylamide hydrolysing activity in sera of human and rabbit was not increased by the oral administration of Pronase-P. (With rats, in some cases, the activity was found to increase.)One of the possibilities that an inhibitor present in serum may cause the enzymic activity to remain within the normal range was biochemically sought. 1) The casein hydrolysing activity of Pronase-P was strongly inhibited by the addition of serum, indicating the presence of an inhibitor in serum. 2) The inhibitory action of serum was not changed by successive oral administration or after introduction of Pronase-P into intestinal loop.3) The casein hydrolysing activity of Pronase-P waes not affectd by the addition of ε-aminocapronic acid. 4) The successive administration of Pronase-P was found to give no pathological change in normal organs. 5) The enzyme activity in serum obtained immediately after injection of Pronase-P was found to corespond to 8-25% of that of the calculated value by assuming the uniform distribution in serum of the injected enzyme.
耳鼻咽喉科臨床学会の論文