Rapid methods for determination of ATP related compounds in muscle by low pressure column chromatography.

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概要

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• The rapid methods for determination of ATP related compounds in muscle by low pressure column chromatography have been studied.<BR>The rapid separation was achieved at a high flow rate of 3 m<I>l</I>/min, by using a Perista Minipump and a small column (φ5 mm×20 mm) packed with an anion exchange resin (AG 1×4, -400 mesh, Cl type).<BR>This made it possible to fractionate ATP related compounds into HxR+ Hx, AMP, IMP, ADP and ATP in about 25 min by the concave gradient elution system. The hydrochloric acid-sodium chloride concentration gradients were prepared with two and three chambered gradient apparatuses.<BR>When the flow rate was reduced to 2 m<I>l</I>/min, the time required for the separation increased to 40 min, but a better separation of each component was obtained at this flow rate.

• 日本食品保蔵科学会の論文

著者

• 内山 均

  東京農業大学

• 宇田 文昭

  (株) 野村生物科学研究所

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