PHOTOLYTIC MICRODETERMINATION OF VITAMIN A IN BLOOD SERUM

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概要

• 論文の詳細を見る

• Purification methods of the reagents used in the Bessey method for vitamin A were improved and simplified. The optical density at 328mμ of kerosenexylene mixture alone was found to rise under the condition of photolysis which makes the correction due to the change in a blank test necessary to determine vitamin A. Under these conditions, vitamin A ester behaves similary to vitamin A alcohol, indicating practically complete saponification of the ester.The values for human serum vitamin A determined by this method were in good agreement with those determined by a micro-GDH-method described in this paper.Carotene is photolyzed under the condition but the effect is insignificant for vitamin A determination. The effect of carotene on GDH method is likewise insignificant usually.

• 日本ビタミン学会の論文

著者

• FUJITA AKIJI

  Biochemical Laboratory, Kitasato Institute

• KIMURA KOJI

  Biochemical Laboratory, Kitasato Institute

関連論文

• FLUOROMETRIC DETERMINATION OF VITAMIN B6:I. DETERMINATION OF PYRIDOXINE
• PHOTOLYTIC MICRODETERMINATION OF VITAMIN A IN BLOOD SERUM
• ISOLATION OF THE THIAMINE DEGRADATION PRODUCTS FORMED BY SHELLFISH THIAMINASE
• THE ISOLATION OF ANILINOTHIAMINE FORMED FROM THIAMINE AND ANILINE BY BACILLUS ANEURINOLYTICUS KIMURA ET AOYAMA
• THE DETERMINATION OF VITAMIN A IN FECES
• THE SYNTHESIS OF VITAMINS BY INTESTINAL BACTERIA IN MAN AND THE EFFECT OF CELLULOSE:I. SYNTHESIS OF RIBOFLAVIN
• THE SECOND TYPE OF BACTERIAL THIAMINASE
• FLUOROMETRIC DETERMINATION OF VITAMIN B6:II. DETERMINATION OF PYRIDOAMINE

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