FLUOROMETRIC DETERMINATION OF VITAMIN B6:I. DETERMINATION OF PYRIDOXINE

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概要

• 論文の詳細を見る

• A fluorometric determination for PIN was described and the effects of various conditions were reported. Under the conditions of the determination, PIN is completely recovered through the entire procedure, PAM, PAL and lactone are practically without effect. PIC itself can interfere remarkably but in the preliminary extraction procedure it is completely converted into its lactone, thus becoming ineffective. For extraction and hydrolysis of tissue PIN, the preliminary extraction at pH 4.5 at 80° for 15 minutes and the successive hydrolysis of the filtered extract in 0.6N H2SO4 at 130° for 1 hour is most suitable in cases of both plant and animal tissues. For neutralizing the acid extract, the partial neutralization by barium hydroxide for removing sulfate ions is beneficial for promoting recovery.

• 日本ビタミン学会の論文

著者

• MATSUURA KANJI

  Biochemical Laboratory, Kitasato Institute

• FUJITA AKIJI

  Biochemical Laboratory, Kitasato Institute

• FUJINO KOHEI

  Biochemical Laboratory, Kitasato Institute

関連論文

• FLUOROMETRIC DETERMINATION OF VITAMIN B6:I. DETERMINATION OF PYRIDOXINE
• PHOTOLYTIC MICRODETERMINATION OF VITAMIN A IN BLOOD SERUM
• ISOLATION OF THE THIAMINE DEGRADATION PRODUCTS FORMED BY SHELLFISH THIAMINASE
• THE ISOLATION OF ANILINOTHIAMINE FORMED FROM THIAMINE AND ANILINE BY BACILLUS ANEURINOLYTICUS KIMURA ET AOYAMA
• THE DETERMINATION OF VITAMIN A IN FECES
• THE SYNTHESIS OF VITAMINS BY INTESTINAL BACTERIA IN MAN AND THE EFFECT OF CELLULOSE:I. SYNTHESIS OF RIBOFLAVIN
• THE SECOND TYPE OF BACTERIAL THIAMINASE
• FLUOROMETRIC DETERMINATION OF VITAMIN B6:II. DETERMINATION OF PYRIDOAMINE

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