FORMATION OF DEOXYINOSINE FROM INOSINE BY WASHID CELL SUSPENSION OF VITAMIN B12-REQUIRING STRAIN OF LACTOBACILLUS LEICHMANNII
スポンサーリンク
概要
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1. The effects of ribonucleosides or deoxyribonucleosides on the growth rate of Lactobacillus leichmannii were investigated using the ordinary microbioassay technique. It was found that the addition of both vitamin B12 and ribonucleosides resulted in higher growth rate than a single addition of vitamin B12. The finding was quite similar to the case where the addition of both vitamin B12 and thymidine was more active than a single addition of vitamin B12. Further, formation of deoxyribonucleoside was observed after addition of both vitamin B12 and ribonucleosides.2. Effects of the age of the cells on the deoxyribonucleoside formation were studied, and the best condition for producing the compounds by washed cell suspension of L. leichmannii was studied.3. 2′-Deoxyribonucleosides were separated from the reaction products and studied chemically and microbiologically. By paper chromatography and bioautography they were separated and identified.4. Formation of 2′-deoxyribonucleosides from the corresponding ribonucleosides with the washed cell suspension of L. leichmannii at the logarithmic phase of the growth rate was studied.
著者
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佐橋 佳一
Laboratory Biochemistry and Nutrition, Tokyo University of Agriculture
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宮沢 栄子
Laboratory of Biochemistry and Nutrition, Department of Agricultural Chemistry, Tokyo University of Agriculture
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前川 昭男
Laboratory of Biochemistry and Nutrition, Department of Agricultural Chemistry, Tokyo University of Agriculture
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鈴木 隆雄
Laboratory Biochemistry and Nutrition, Tokyo University of Agriculture
関連論文
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