ISOTOPIC STUDIES ON 2′-DEOXYINOSINE-8-C14 FORMATION FROM INOSINE-8-C14 BY WASHED CELL SUSPENTION OF VITAMIN B12-REQUIRING STRAIN OF LACTOBACILLUS
スポンサーリンク
概要
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1. Isolation and confirmation of 2′-deoxyinosine and related compounds which appeared in the reaction mixture were made by two-dimensional ascending paper chromatography, and each spot was applied to bioautography, autoradiography and chromatoscanning. The recoveries of the standard compounds previously added to the reaction mixture were satisfactory.2. Formation of 2′-deoxyinosine-8-C14 from inosine-8-C14 In the presence of vitamin B12 was tested by two-dimensional paper chromatography, and paper chromatoscanning, and the labeled deoxyinosine was detected in the amount of 8% of the added C14.3. Using the similar method, the incorporation of the labeled carbon of hypoxanthine-8-C14 or D-ribose-1-C14 in deoxyinosine was studied. In the presence of hypoxanthine-8-C14, D-ribose and CN-B12, the labeled carbon was found in the deoxyinosine fraction by paper chromatoscanning in the amount of 2.7% of the added C14, but in the presence of hypoxanthine, D-ribose-1-C14 and CN-B12, the incorporation of the labeled carbon into deoxyinosine was scarcely detected.
著者
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佐橋 佳一
Laboratory Biochemistry and Nutrition, Tokyo University of Agriculture
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宮沢 栄子
Laboratory of Biochemistry and Nutrition, Department of Agricultural Chemistry, Tokyo University of Agriculture
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前川 昭男
Laboratory of Biochemistry and Nutrition, Department of Agricultural Chemistry, Tokyo University of Agriculture
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盛永 宏太郎
Laboratory of Biochemistry and Nutrition, Department of Agricultural Chemistry, Tokyo University of Agriculture
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鈴木 隆雄
Laboratory Biochemistry and Nutrition, Tokyo University of Agriculture
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