Molecular cloning of immunoglobulin gamma gene from a patient with myeloma.
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Messemger RNA was purified from the tumor at the distal end of right clavicula of the myeloma patient.The concentration of gamma chain in the total polyA+ RNA was estimated as about 1% by hybridization. Under the restriction of P2 level, cDNA library was constructed using Amersham's kits. The efficiency of the cloning reached to 4.5×107 pfu per μg cDNA. Seven positive plaques from 500 plaques were picked up and purified. Each clone had an about 300bp insert which was hybridized to the probe.
- 西日本整形・災害外科学会の論文
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