A rapid method for detection of cytotoxicity of metal using carboxyfluorescein. Second Report.
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A cytotoxicity test of biomaterials (nickel, chromium, and iron) using carboxyfluorescein diacetate (CFDA) was performed with rat fetal lung fibroblast (RFL-Fb). Cytotoxicity of chromium and nickel on viability of RFL-Fb was severe and the number of viable cells was decreased dose-dependently. However, cytotoxicity of iron on RFL-Fb was not detected. This method might be reliable to detect cytotoxicity of biomaterials on various kinds of cells.
- 西日本整形・災害外科学会の論文
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