The Distribution of Motoneuros Corresponding to Cervical Nerve Roots in the Rat. A Study using the Horseradish Peroxidase(HRP) method.:A Study using the Horseradish Peroxidase (HRP) method.

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We investigated the distribution of spinal motoneurons corresponding to cervical nerve roots using the HRP (horseradish peroxidase) method as well as assessing the anatomical levels of anterior and posterior filaments, and discs. Eighteen forelimbs of Wistar rats were used, and 250I. U. (3μl) HRP solusion was injected into their cervical nerve roots. The levels of anterior and posterior filaments and discs on the corsd were noted microscopically, and motoneurons in the anterior horn at each levels were labelled, then compared to the levels of anterior and posterior filaments and discs.Motoneurons corresponding to the C5 nerve root were distributed from the middle of the C4 segment to upper part of the C6 segment with 92.3% of motoneuros located in the C5 segment.Motoneurons corresponding to the C6 nerve root were distributed from the middle of the C5 segment to the upper part of the C7 segment with 95.8% of motoneurons located in the C6 segment.Motoneurons corresponding to the C7 nerve root were distributed from the upper part of the C7 segment to the middle of the C8 segment with 95.5% of motoneurons located in the C7 segment.Anterior and posterior filament levels were almost the same from C5 to C7 and C4/5, C5/6, C6/7 disc levels were almost the same C5/6, C6/7, C7/8 spinal segments.