海洋細菌の無機塩要求性に関する生理学的研究-9-14C-コハク酸,14C-アラニンおよび14C-グルコ-スの細胞内取込みにおける無機塩の役割
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The effects of various inorganic salts, 2, 4-dinitrophenol (2, 4-DNP), KCN and ouabain (g-strophanthin) on the incorporation of 14C-succinic acid, 14C-alanine, and 14C-glucose into the cells of M(marine)-, MH(marine halophilic), TH(terrestrial halophilic)-, and T(terrestrial)-types were examined in the Warburg respirometer, and the oxidating activities measured. The strains of M-, MH-, and TH-types had an essential requirement for a definite amount of Na+, the function of which in the incorporation of 14C-succinic acid and 14C-alanine could not be mimicked by Li+, Mg2+, or Ca2+. The sparing effects between Na+ and K+ were observed but the function of K+ was not displaced by Li+. The T-type strain needed trace supplements of Na+ and K+ in order to incorporate either substrate. The larger portion of the incorporated substrate was metabolized to 14CO2 and the residual substrate was retained within the cells. This fact was more obvious in the case of the M- and MH-types. Such a Na+, K+-dependent incorporation was blocked by 2, 4-DNP and KCN but not by ouabain. The correlation between the incorporation and the oxidizing activity was observed under various conditions. These results show that succinic acid and alanine permeate into the cells by means of the Na+, K+-dependent active transport utilizing the electron transport chain related ATP. In contrast to succinic acid and alanine incorporation, that of 14C-glucose was not affected either by inorganic salts of by 2, 4-DPN and ouabain in any of the strain types and no correlation between the incorporation and the oxidizing activity was observed. These results suggest that glucose is transported into the cells by the phosphoenol-pyruvate-glucose transferase system. Based on the findings described in these nine serial reports, the differences in the physiological roles of the monovalent cations (Na+ and K+) and the divalent cations (Mg2+ and Ca2+) among the M-, MH-, TH-, and T-types were discussed. The authors concluded that type-specificity among the M-, MH-, TH-, and T-types and its theoretical basis regarding this typing method were clarified by this biochemical and physiological study and this method, therefore, provided a tool for discriminating marine bacteria from non-marine bacteria.
- 日本水産學會の論文
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