結核感染宿主の代謝の変動

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In the preceding paper (Amer. Rev. Resp. Dis., 94: 625, 1966), one of the writers reported the elevation of NAD-ase activities of liver, kidney, spleen, and lung of guinea-pig infected with tubercle bacilli. In this paper, the metabolic changes of tuberculous mice were reported. Mice were infected with tubercle bacilli intravenouslly and enzymatic activities of their tissue homogenates were observed successively following the course of the infection. NAD-ase was assayed according to the method of Kaplans KCN method. Acid phosphatase was assayed with <I>p</I>-nitrophenylphosphate as substrate. Succinate dehydrogenase was assayed with triphenyltetrazolium chloride as electron acceptor.<BR>As shown in Fig.1, the enhancement of NAD-ase activity was observed by tuberculous mice as well as tuberculous guinea-pigs, but the degree of the elevation was much less remarkable by the former than by the latter. Repeated experiments under the same experimental conditions gave the results with fairly good reproducibility as shown in Fig.2. NAD-ase, acid phosphatase and succinate dehydrogenase activities were all enhanced by the tuberculous infection, but their variation patterns were linkely to be independent each other (Fig.3 and 4). The degrees of metabolic changes were compared, after several strains of tubercle bacilli with different virulence were used for the infection. As shown in Fig.5-7, the degree of metabolic enhancement were not found to be proportional to the degree of virulence.<BR>From these experimental results, the writers discussed some plausible m echanism of NADase enhancement by tuberculous infection. Also, the writers stressed the necessity to follow the whole course of the infection as possible to understand the biochemical changes of the infected host.
日本結核病学会の論文

結核感染宿主の代謝の変動

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