経気管感染によるマウスM. intracellulare症実験モデル作成の試み
スポンサーリンク
概要
- 論文の詳細を見る
A trial to induce Mycobacterium intracellulare infection in white conventional mice suit able for the evaluation of experimental chemotherapy was presented. Mycobacterium intracellulare, TMC 1469 strain (provided by the U.S.-Japan Cooperative Medical Science Program NIAID in 1975), grown in modified Dubos Tween albumin liquid medium for ten days was used for intratracheal instillation. For comparison, Mycobacterium tuberculosis (H37Rv strain) was also inoculated to mice by the same method.<BR>Each inoculum was administered by a syringe into the cervical trachea of the mouse which had been slightlyanesthetized with cholroform and secured supine to expose cervical trachea through a small skin incision. Mice were sacrificed at two days, one week, three weeks, six weeks, nine weeks, twelve weeks and twenty weeks after the infection and quantitative cultures as well as histopathological examinations of lungs, spleen, liver and kidney were performed.<BR>Seven mice infected with M. intracellulare and eighteen mice infected with M. tuberculosis died during the experimental period with a mortality rate of 13.5% and 31.6%, respectively. Increase of body weight was suppressed until three weeks after M. intracellulare infection and until nine weeks after M. tuberculosis infection, when compared to those of mice which admin istered either Dubos Tween albumin liquid medium or normal saline by the same route. In the mice infected with M. intracellulare, the viable units of bacilli in 10mg of lung was 2.9×10<SUP>5</SUP> at the second day, 4.5×10<SUP>4</SUP> at the third week and 2.7×10<SUP>2</SUP> at the twentieth week after the infection, and failed to show a phase of logarithmic multiplication which was seen in the mice infected with M. tuberculosis between the first to third week (1.0×10<SUP>4</SUP> to 8.4×10<SUP>6</SUP>v.u.) after the infection. Histopathological changes became remarkable three weeks after the infection. In the mice infected with M. intracellulare, fairly extensive infiltrations of mononuclear cells in the lungs were observed in approximately one half of the mice and epithelioid cell granulomas in the lungs were seen in four mice. While, in the mice infected with M. tuberculosis, almost all the mice sacrified between the third to twentieth week after the infection showed severe extensive infiltrations of mononuclear cells and macrophages in the lungs, as well as epithelioid cell granulomas in the liver which were not recognized in the mice infected with M. intracellulare.<BR>By this intratracheal instillation method, it was possible to administer a large size of in oculum of M. intracellulare to lungs, and the viable units of bacilli in the lungs were higher than the viable units of bacilli in spleen. In this regard, the intratracheal route of infection was thought to be superior to the ordinary intravenous route. However, in view of these results, it was again evident that M. intracellulare is considerably less virulent than M. tuberculosis. Studies on colony morphology of M. intracellulare might contribute to obtain virulent strains. Further endeavors and mandatory to obtain a suitable experimental model of M. intracellulare infection.
- 日本結核病学会の論文
著者
関連論文
- 経気管感染によるマウスM. intracellulare症実験モデル作成の試み
- 実験的非定型抗酸菌症に関する研究 : 5. Mycobacterium kansasii感染マウスに対する抗結核薬の併用治療効果
- 実験的非定型抗酸菌症に関する研究 : 4. Mycobacterium kansasii感染マウスに対する抗結核薬の単剤治療効果
- 諸種消毒剤の結核菌に対する殺菌効果
- 実験的非定型抗酸菌症に関する研究 : 3. マウスを対象としたMycobacterium intracellulare吸入感染の試み
- 実験的非定型抗酸菌症に関する研究-6-実験的Mycobacterium fortuitum症作成と治療の試み