諸種消毒剤の結核菌に対する殺菌効果

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The bactericidal activities of various disinfectants against Mycobacterium tuberculosis were evaluated by two methods. The bactericidal effects of these agents against M. tuberculosis (H37Rv) were studied with Silicone-coated Slide Culture Method. The silicone-coated slides, which had been immersed into approximately 1.0mg/ml of bacillary suspension (H37Rv) in petroleum benzine, were placed in serially diluted solutions of the various disinfectants. After various intervals from one minute to twenty four hours, the slides were removed and washed in the sterile normal saline to discontinue the action of the disinfectants. The slides were then cultured in Kirchners liquid medium with ten percent bovine serum. After eight weeks of culture, all slides were examined for the growth on the surfaces of the slides. Some of the disinfectants were further evaluated using sputum specimens obtained from the patients with pulmonary tuberculosis who had positive smears. The sputum specimens, which had been pretreated with two percent sodium bicarbonate, were mixed with equal amounts of the disinfectants of various concentrations. After ten minutes to twenty four hours interaction, quantitative cultures were made on one percent Ogawa media and relative potencies of the disinfectants were observed with the time-kill relationships. Saponated cresol solution showed the strongest bactericidal activity against M. tuberculosis (H37Rv) and the bacilli in the sputa. Ten percent of saponated cresol solution killed M. tuberculosis (H37Rv) on the surfaces of the slides in one minute, and the bacilli in the pretreated sputum specimens (1.3×106v. u./ml) with the ultimate concentration of five percent in less than ten minutes. The activities of two kinds of ampholytic surface active agents (Vista #300®, Tego 51®) were approximately parallel to saponated cresol, though the latter was more potent than the former in less than one hour. Iodophors (Isocline®, MK 212®) needed the higher concentrations to kill the bacilli in the sputa in spite of almost the same bactericidal activities as saponated cresol against H37Rv on the slides. Two percent glutaraldehyde (Sterihyde®) required at least thirty minutes to kill M. tuberculosis (H37Rv). Ten percent benzalkonium chloride solution (Osvan®) and five percent chlorhexidine gluconate solution (Hibitane®) showed no bactericidal activities against M. tuberculosis.
日本結核病学会の論文

著者

李英徹
京都大学結核胸部疾患研究所内科学第I

諸種消毒剤の結核菌に対する殺菌効果

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