マウス気管支より分離したマイコプラズマ-2-液体培地を用いての終未価測定

概要

論文の詳細を見る
Strain mA of Mycoplasma pulmonis, one of the isolates from the bronchi of normal mice, grew readily in the tissue culture medium (YLE) currently used at the authors' laboratory. Since this strain was known to utilize glucose, an attempt was made to titrate it in YLE by the aid of color change of phenol red which had been added to the medium as an indicator. The full titer determined by this method was comparable with that by the conventional colony counting method. The period required to obtain an end point was 3 or 4 days in the new method, while it was 1 week in the conventional method. A serum concentration of 2% was enough to obtain the full titer. Without serum, however, no color change occurred during an observation period of 2 weeks. A starting pH of 7.2 was proved to be the most sensitive in the assay system. When YLE (pH 7.2) was compared with Chanock's PPLO broth, the superiority of the former was evident, since the period required for the end point to appear was between 3 and 4 days in the former and between 5 and 6 days in the latter. It was possible to detect a single growth unit from a material by the method with YLE, so long as the material had been filtered through an HA type of millipore filter.
日本細菌学会の論文