酵母様真菌細胞微細構造の電子顕微鏡的研究-1-Candida albicans,Candida stellatoideaのChlamydosporeの微細構造について

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The purpose of the present study is to obtain a knowledge concerning the fine structure of chlamydospore in genus Candida (C. albicans and C. stellatoidea) which has not been manifested sufficiently.The chlamydospore could be grown on the surface of the medium for chlamydospore formation abundantly that is composed of cornmeal extract, liver extract, starch and Tween 80. The cells were prepared as the materials for electron microscopy.Specimens are fixed in a 2% potassium permanganate and a 1% osmium tetroxide, after serial alcohol dehydration, emedded in an epoxyresine mixture.In order to obtain the best materials demonstrating the fine inner structure of chlamydospore cells. The materials sould be fixed in a 2% solution of potassium permanganate, buffered at pH 7.0 with phosphate buffer (at 0-2°C for 12-20 hours) than with an osmium tetroxide.The results obtained were as follows.The cell wall consists of the three layers. The outer and inner layers of cell wall are composed of electron dense materials and intermediate layer is lesser electron density. Furthermore, the inner layer which keep possession of greater part of cell wall, about 200-300mμ in average thickness, could be observed to laminated.The boundary of the three layers of cell wall are observed more definite structure in chlamydospore of C. albicans than in chlamydospore of C. stellatoidea.The cytoplasmic membrane, very thin, is observed in the mature spore but could not observed in the immature cell.The nucleus is bounded by a double membrane with pore, it exists near the peripheral region of cytoplasm. The nucleolus like structure are observed as an electron dense region in the nuclearplasm.The mitochondria with mitochondrial tubules are also present in peripheral region of cytoplasm in mature chlamydospore. The mitochondria bounded by a double membrane and inner mitochondrial tubules are also composed of parallel lamellae.The endoplasmic reticulum could not observed in the fully developed cell, as the alternative, the vacuole structure occupies the greater part of cytoplsm which is characterristic of a spongoid structure and the othre include less dense plain matrix in it.
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酵母様真菌細胞微細構造の電子顕微鏡的研究-1-Candida albicans,Candida stellatoideaのChlamydosporeの微細構造について

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