抗酸菌の溶原化に関する研究 : 第2報 Y13L溶原獣調の示すautoplaqueとdismune現象

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概要

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• It was found that “auto-plaques” were produced without addition of any indicatorstrain on the lawn of Myc. Jucho, which was preliminarily lysogenized by Y 13 L phage, subcultured on Ogawas egg slant and seeded on agar plate, and that the bacterial growth was inhibited in the same time, on the similarly seeded agar plate with the same Mycobacterium, by spotting a phage suspension of Y 13 L. However, no plaquewas produced on agar plate or Ogawas egg plate seeded with the same bacterial culture maintained on agar media.<BR>Turbidity of the “auto-plaque” depended on the culture age of the lysogenic strainof Ogawas egg slant from which bacterial suspension seeded was prepared, namely, the older became the culture, the clearer became the plaque.<BR>Addition of Calcium chloride at a cencentration of 2x10<SUP>-3</SUP> Moliml to Ogawas madia, on which the bacteria was subcultured, inhibited the “auto-plaque” formation completely.<BR>Analytical studies suggestedthat the “auto-plaque” and “dismune” phenomena described above were produced by neither a virulent mutant from Y 13 L phage nor a sensitive mutant population derived from lysogenic Jucho.<BR>The number of colonies produced on Ogawas egg plate by seeding a bacterial suspension of the lysogenic Jucho prepared from Ogawas slant was about three times as much as that of the agar plate.<BR>These investigations seem to suggest thepossibility of phage induction of lysogenic Jucho strain by changing the culture media from Ogawa to agar.

• 日本細菌学会の論文

著者

• 水口 康雄

  国立予防衞生研究所結核部

関連論文

• 抗酸菌の溶原化に関する研究 : 第1報 Y13ファージによる抗酸菌獣調株の溶原化
• 抗酸菌の溶原化に関する研究 : 第2報 Y13L溶原獣調の示すautoplaqueとdismune現象
• 溶原性抗酸菌の誘発に関する研究
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