抗酸菌フアージの吸着に関する研究 : (第1報) 十二種の系におけるフアージ吸着率流産率, 増殖, およびそれらに対するTween 80の作用について

概要

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Quantitative studies were made on the EOP, adsorption rate, abortion rate, adsorption velocity constantand propagation rate, using 3 mycophages and 4 mycobacterial strains. The adsorption velocity of mycophage was found to be rather slow, and the velocity constant calculatedwas in the order of 10-10-10-11. Under the present experimental conditions, only two among 12 systems have shown the adsorptionrates of more than 90% after 60 minutes adsorption. Rather high abortion rate was found in a few systems, especially in D 29-Myc. Phlei-yoken system.Namely, in this system, all of the phage particles which adsorbed to nearly 90% to bacterial cells wereabortive. Generally, the system, in which the difference between adsorption and abortion rates was big, seemedto have a good efficiency of phage propagation. For instance, the system of HC-Myc. sp. jucho had thelargest propagation rate, in spite of its relatively low adsorption rate. Tween 80 inhibited the adsorption and the propagation of mycobacteriophage in all 12 systems.
日本細菌学会の論文