細菌のChloramphenicol不活作用に関する研究 : 第3報大腸菌のChloramphenicol不活作用に及ぼす各種の影響ならびにChloramphenicolの分解について

概要

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Since the chloramphenicol inactivation by E. coli had been found markedly in the natural and transferred resistant strains, the action of the resistant E. coli on chloramphenicol was examined by the cup. plate assay. It was very active at 40-45°C and after 4hours, 50μg per ml of chloramphenicol werecompletely inactivated by the bacterial suspension. The action of bacteria was destroyed after heating at 60°C for 30minutes and was markedly stimulated by low concentration of glucose and peptone. The substance concerned with the action was intracellular and spheroplasts of the bacteria produced by penicillin, glycine and LiCI, also presented the activity.<BR>From the infrared spectra on the decomposition products, it appeared that the major pathway employed by the resistant <I>E. coli</I> for decomposition of chloramphenicol would be an oxidative process
日本細菌学会の論文