Characteristics of tissue ultraweak chemiluminescence in rats fed with autoxidized linseed oil.

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概要

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• After 2 days of oral administration of autoxidized linseed oil (AOLO; peroxide value 1, 000meq/kg, 0.3ml/rat/day), ultraweak chemiluminescence (CL) spontaneously emitted from rat liver homogenate increased up to about three times accompanying the accumulation of thiobarbituric acid (TBA) reactive products. This liver CL was quenched effectively by the in vitro addition of free radical scavengers, e.g. butyl hydroxytoluene, d-α-tocopherol and 2, 5-diphenylfuran, while superoxide dismutase, catalase and D-mannitol gave only negligible inhibitions on AOLO-induced liver CL. The luminescence spectrum recorded by a filter spectral analyzing method gave definitive two emission peaks at 585 and 635 nm and broad emission band around 500-560 nm. The spectrum was similar to that reported for a singlet molecular oxygen. In the experiment of successive AOLO administration (0.3ml/rat/day) for 10 days, a large increase in CL intensities and TBA reactants in various tissues was observed, especially on the 2nd day to the 6th day of feeding. Among them, the enhancement of CL and the accumulation of TBA reactants in liver and kidney were inhibited significantly by the oral administration of supplemental d-α-tocopherol (5mg/rat/day). These results suggest that AOLO-induced tissue spontaneous CL originated in the occurrence of free radical species which are possibly derived from chain reactions of tissue lipid peroxidation caused by the AOLO administration to rats.

• 財団法人 学会誌刊行センターの論文

著者

• 宮沢 陽夫

  Department of Agricultural Chemistry, Obihiro University

• 金田 尚志

  Department of Food Chemistry, Faculty of Agriculture

• 稲場 文男

  Research Institute of Electrical Communication, Tohoku University

• 宮沢 陽夫

  Department of Food Chemistry, Faculty of Agriculture

• 田久 長一

  Research Institute of Electrical Communication, Tohoku University

• 金田 尚志

  Department of Food and Nutrition, Kohriyama Women's College

関連論文

• FATTY ACID COMPOSITION OF GLYCERIDES AND STEREOSPECIFIC ANALYSIS OF TRIGLYCERIDE IN PEA SEEDS
• Characteristics of tissue ultraweak chemiluminescence in rats fed with autoxidized linseed oil.
• Hypocholesterolemic effect of triterpene alcohols with soysterol on plasma cholesterol in rats.
• Extra-weak chemiluminescence of organ homogenate and blood in tocopherol-deficient rats.
• Decreases in spleen weight and blood leucocytes number with long-term feeding of oxidized oil in mice.
• Spontaneous and luminol-dependent chemiluminescences from tissue preparations of benzo(a)pyrene-injected mice.

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