Membrane-to-membrane transfer of tocopherol in red blood cells.
スポンサーリンク
概要
- 論文の詳細を見る
The direct cell-to-cell transfer of tocopherol between red blood cells (RBCs) was examined. Two kinds of RBCs were provided; 1) Tocopherol-deficient ones showing complete dialuric acid-hemolysis and 2) tocopherol-supplemented ones showing no dialuric acid-hemolysis. The two kinds of RBCs were mixed and incubated with gentle swirling in a buffer solution. If no tocopherol transfer occurred between the two kinds of RBCs in the cell suspensions, hemolysis should not change during incubation because hemolysis is limited only in the -deficient cells in the suspensions. However, it was actually observed that when RBCs with adequate amounts of tocopherol were incubated with tocopherol-deficient RBCs, dialuric acid-induced hemolysis decreased during a 3-h incubation period. Contrarily, when an inadequate amount of tocopherol constitut-ing a limiting level for inhibition of hemolysis, exsisted in RBCs which were mixed with -deficient ones, hemolysis increased after incubation. This indicates that tocopherol is transferred from the tocopherol-rich RBCs to the -deficient RBCs. The transfer was greater as the hematocrit of cell suspensions increased. Gum arabic contained in the suspensions inhibited the transfer, while bromelain (a protease which lowers the electric charge on the cell surface) increased it. These findings indicate that the transfer of tocopherol is related to the frequency of collision between cells.
- 財団法人 学会誌刊行センターの論文
著者
-
美濃 真
Department of Pediatrics, Osaka Medical College
-
田中 英高
Department of Pediatrics, Osaka Medical College
関連論文
- Serum Free Amino Acid Depletion in Ketotic Children
- Effect of Body Lipid Mass on Red Blood Cell-and Plasma-Tocopherol Levels
- FETAL AND MATERNAL RELATIONSHIP IN SERUM VITAMIN E LEVEL
- Adipose Tissues and Vitamin E
- OXYGEN POISONING AND VITAMIN E DEFICIENCY
- Platelet Tocopherol Levels in Mucocutaneous Lymph Node Syndrome (MCLS: Kawasaki Disease)
- Free Radical Chain Oxidation and Hemolysis of Erythrocytes by Molecular Oxygen and Their Inhibition by Vitamin E
- Changes of Alpha-Tocopherol Levels in Red Blood Cells and Plasma with Respect to Hemolysis Induced by Dialuric Acid in Vitamin E-Deficient Rats
- Studies on the factors influencing the hydrogen peroxide hemolysis test.
- Tocopherol level in human blood cells.
- Difference in plasma- and red blood cell-tocopherols in breast-fed and bottle-fed infants.
- Free radical-mediated damage of blood and its inhibition by antioxidants.
- A nutritional evaluation of vitamin E status in very low birth weight infants with respect to changes in plasma and red blood cell tocopherol levels.
- Galactose absorption after oral administration of lactose in neonates.
- Alpha-tocopherol and fatty acid levels in red blood cells in patients treated with antiepileptic drugs.
- Chemiluminescence in vitamin E-deficient erythrocytes initiated by xanthine oxidase reaction, in relation to the accumulation of thiobarbituric acid reactive substances.
- Comparative changes in plasma and RBC .ALPHA.-tocopherol after administration of dl-.ALPHA.-tocopheryl acetate and d-.ALPHA.-tocopherol.
- Effects of elevated d-alpha (RRR)-tocopherol dosage in man.
- Membrane-to-membrane transfer of tocopherol in red blood cells.
- Simple method of tocopherol determination in plasma lipoprotein fractions using a heparin-Ca precipitation technique.
- Tocopherol concentration in platelets in normal children and adults.