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The inhibitory effect of ethanol fraction in the supernatant of F. nucleatum KO-31 culture on cancer cells was examined by in vitro-in vivo method. When 4 mg/ml of ethanol fraction was given to mice previously transplanted 4.0×107 cells/ ml of Ehrlich ascites, a remarkable antitumor activity was observed with T/C (%) of 197.0% or more. Following the administration of ethanol fraction at an increased dose of 12 mg/ ml to mice bearing 4.0 ×107 cells/ ml of Sarcoma 180, an antitumor activity was also noted with T/C (%) of 154.5% or more. On the other hand, whether or not this experiment produced any cytomorphological changes was examined under light microscope. Control cells pre-cultured for 180 min were all round or elliptrical having large nucleus, distinct nucleoli, and a small number of lipid granules in cytoplasma, while a vacuolation and destruction of cells were observed in ethanol fraction treated cells in addition to the degeneration and disappearance of cell membrane and extracellular outflow of protoplasma. A direct antitumor action was demonstrated to exist in the supernatant of F. nucleatum KO-31 culture not only from the basic experiment but also from the light microscopic observation.
社団法人日本口腔外科学会の論文