ヒト唾液腺初代培養細胞の増殖性に及ぼすイソプロテレノールの影響

概要

論文の詳細を見る
The effects of isoproterenol on the outgrowth from human salivary gland explants were studied in primary culture. Isoproterenol was added to the growth medium to produce a graded series of concentrations of 10-4, 10-5, 10-6 and 10-7g/ml. In the absence of isoproterenol, the cellular outgrowths from salivary gland were easily degenerated after 4-6 weeks of culture, and in most cases fibroblastic growth exceeded epithelial outgrowth. Isoproterenol did not increase the growth of salivary gland cells when used at doses of 10-4, 10-5 and 10-6g/ml. At 10-5g/ml, isoproterenol appeared to have the property of maintaining the sheet-like structure of cellular outgrowth. The inhibitory effect on fibroblastic growth was observed in doses of 10-4, 10-5 and 10-6g/ml. These results show that isoproterenol may act as a regulatory factor in the control of the proliferation and differentiation of human salivary gland cells.