ヒト歯肉部培養上皮細胞における細胞骨格の分布

概要

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In order to identify the organization of cytoskeletons in cultured oral keratinocytes and to characterize the changes of these filamentous systems in oral mucous diseases, cultures of oral keratinocytes from gingiva were investigated by immunofluoresence microscopy, using monoclonal antibodies to keratin, α-tubulin and actin.The cultures were fed with Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum, 0.4 ng/ml hydrocortisone, 10 ng/ml epidermal growth factor, 84 ng/ml cholera toxin, 100 ng/ml streptomycin and 100 unit/ml penicillin.The arrangement of keratin intermediate filaments (KIF) in these cells showed a dense meshwork around the nucleus and a radiating pattern of filament bundles to the cell periphery.Bundles of KIF ended at desmosomes. Microtubles also showed an array similar to that of keratin but these bundles of microtubles ended freely at the cell periphery independently from those of the neighbouring cells. The fluoresence pattern of actin showed diffuse distribution throughout the cell and filamentous structures were not observed.After treatment with cytochalasin B, KIF were rearranged into a "Lattice" structure, but no significant re-organizations were observed by colchicin treatment. These results demonstrate that organization of cytoskeletons in oral keratinocytes is very similar to that of skin keratinocytes.
社団法人日本口腔外科学会の論文