n-パラフィン資化性酵母の分離方法

概要

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In order to isolate n-paraffin assimilating yeasts grown at 37°C from soils, three isolating methods were carried out and compared. They were plate culture method, conventional enrichment culture method using shaking flask and method of enrichment culture using 20l jar fermenter in which 50 samples of soil were added simultaneously. In plate culture method, soils were dried in room temperature and scattered on plate and yeast colonies grown on the plate were isolated. Although it was comparatively easy to isolate n-paraffin assimilating yeasts by the plate culture method by which 39 strains were obtained, the cell growth of the yeasts in an n-paraffin medium was generally poor. By enrichment culture method using shaking flask, 234 strains were isolated. Some of them showed either good or poor growth. However, many of them gave moderate cell production in the n-paraffin medium. Enrichment culture method using jar fermenter was found to be the best for isolating yeasts assimilating n-paraffin strongly. Eleven strains were isolated by this method and all of them showed good cell growth.
社団法人日本農芸化学会の論文