カラムライトを担体とするカラムクロマトグラフィーによる塩基性抗生物質の吸着分別

概要

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Column chromatography of several basic antibiotics was examined by using columnlite, which is an uniform bead (30_??_60 mesh) composed of Al2O3•MgO•2 SiO2; XH2O. Basic oligosaccharide group antibiotics and basic peptide group antibiotics were adsorbed onto column-lite at neutral pH range and eluted from it by increasing the concentration of sodium chloride or ammonium bicarbonate in the eluate, or by dilute aqueous ammonia solution. Such adsorptive capacity was hardly affected by the presence of proteins and color impurities. These results indicate that column-lite may be very useful for the purification of basic antibiotics from the culture filtrate of microorganisms and also for analysis of the antibiotics remaining in blood and urine after the clinical administration.
社団法人日本農芸化学会の論文