Effect of bile acids on the precipitation of calcium bilirubinate.

概要

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Bile acid-calcium mixtures were made by mixing conjugated bile acids at different concentrations with CaCl2 solution. The centrifuged supernatants of each bile acid-calcium mixture were divided into two fractions using ultrafiltration technique (fraction molecular weight 1, 000). Both fractions and the supernatants were tested for the inhibitory effect on bacterial β-glucuronidase activity, the calcium concentration and the bile acid concentration. Almost all bile acids were present in a high molecular phase and they had a high calcium trapping capacity, except glycocholate and taurocholate. The highest inhibitory effect on bacterial β-glucuronidase activity and calcium trapping capacity were obtained with chenodeoxycholate and deoxycholate, and the calcium trapping capacity was the lowest with taurocholate. The bile with calcium bilirubinate stone has a markedly low concentration of conjugated bile acids but with a relatively high concentration of taurocholate, which produces the favourable condition for the precipitation of calcium bilirubinate.
財団法人日本消化器病学会の論文