Protoplast culture and plantlet regeneration in stock (Matthiola incana R. Br.).

概要

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Protoplasts were isolated from callus derived from excised cotyledons of stock (Matthiola incana R. Br.), by using an enzyme mixture of 1.5% Meicelase and 0.05% Macerozyme R-10. Modified Murashige-Skoog major elements, Ringe and Nitsch minor elements and modified vitamins supplemented with 0.2mg/l 2, 4-D, 1mg/l NAA, 1mg/l BAP, 8.1% mannitol, 1% glucose and 1% sucrose supported protoplast division at 25°C. The frequency of colony formation 10 days after culture was the highest (17.9% of isolated protoplasts) at protoplast density of 0.5×105/ml. Colonies developed to the callus-stage 2 months after initial culture. When callus pieces were cultured in full strength MS medium supplemented with 1mg/l BAP or 1mg/l zeatin, adventitious buds were induced in 9%-16% of callus explants. The buds elongated with leaves open at 20°C and rooted in medium supplemented with 0.1mg/l or 5mg/l IBA.
日本植物細胞分子生物学会の論文