気相シークエンサーを用いたアミノ酸配列分析

概要

論文の詳細を見る
A gas-phase protein sequencer which has been developed for last several years allows the determination of <I>N</I>-terminal amino acid sequence of 2030 residues from picomole amounts of proteins. Recently, using the gas-phase sequencer, a new technique for protein microsequence analysis has been established. In this method, picomole amounts of proteins are first separated by gel electrophoresis, then electroblotted from the gel onto sequencer stable membrane supports, and finally sequenced directly with the gas-phase sequencer. This method frequently permits sequence determination of proteins that could not be easily purified by conventional column chromatographies. In this review, the new instrument and technique for protein microsequence analysis are described.
社団法人日本油化学会の論文