高速液体クロマトグラフィーの化粧品分析への応用 (第7報) : 逆相分配クロマトグラフィーによるアシル化アミノ酸型アニオン及びアルカノールアミド型非イオン界面活性剤の親油基分離

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Separation of homologous series of standard sodium N-acylated sarcosinates and fatty acid mono-isopropanol-amides with C12, C14, and C16 alkyl groups were studied by high speed liquid chromatography, employing octadecylsilica (TSK-Gel; LS-410, 5μm) as a column packing.<BR>The recommended conditions for the separation were as follows : column size, 4mm i.d.×25cm; eluent, water-methanol (15 : 85, vol/vol) adjusted to pH 2.2 with phosphoric acid; column temp., 50°C; detector, UV (210nm).<BR>The capacity factor (k) values of homologous series of sodium N-acylated sarcosinates were drastically affected with the eluent pH; the k values increased with lowering the eluent pH. However, fatty acid monoisopropanolamides were not affected with the eluent pH. The logarithum values of the k were directly proportional to the alkyl chain length of the standard surfactants.<BR>By using the same eluent conditions, other commercial N-acylated amino acid salts (anionic sur-factants) such as mono sodium N-acylated glutamates, sodium N-acylated-N-methyl taurates, and fatty acid alkanolamides (nonionic surfactants) such as fatty acid mono- and diethanolamides could be easily separated to their corresponding homologous series. Typical separation of coconut and hydrogenated tallow fatty acid derivatives were described in this paper.
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