ブリ油について (第3報) : 肝臓の複合脂質について
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(1) The characteristics of the various lipids (acetone-soluble lipid, lecithin, cephalin and sphingolipid) contained in the liver of yellowtail, <I>Seriola quinqueradiata</I>, were studied from the view point of lipid metabolism of fish.<BR>(2) Fresh livers of the fifty five fish caught in the Koyawata fishing ground near Odawara City (west part of Kanagawa Prefecture, Japan) on the 12th of April, 1959 were extracted with acetone. The acetone extract was further treated with acetone to yield the fatty oil as acetone-soluble lipid.<BR>(3) The acetone-soluble lipid was obtained at yield of 8.84% to the fresh livers.<BR>(4) Lecithin purified from the alcohol soluble fraction was obtained at yield of 0.22% to the fresh livers. Spectrophotometry of the alkali-isomerized liquid acids indicated the presence of conjugated tri- to pentaenoic acids in the lecithin. According to rough calculation, composition of the conjugated fatty acids of the lecithin in the livers seems to consist of 34.98% saturated and 65.02% unsaturated acids.<BR>(5) Cephalin purified from the alcohol insoluble fraction was obtained at yield of 0.72% to the fresh livers. Fractional precipitation of the purified cephalin from chloroform with absolute ethanol yielded five groups and showed the presence of phosphatidyl serine, phosphatidyl ethanolamine and inositol phosphatide in the cephalin. Spectrophotometry of the alkali-isomerized liquid fatty acids showed no presence of polyenoic acids. According to simple calculation the conjugated fatty acids of the cephalin in livers consist of 30.13% saturated acids and 69.87% unsaturated acids. The paper chromatography indicated the presence of myristic, palmitic, stearic, zoomaric, oleic, erucic, linolic and linolenic acids in the lecithin and cephalin obtained from the livers.
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