On Diluent and Dilution Method in Hemagglutination Inhibition Test for Human Chorionic Gonadotropin
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概要
- 論文の詳細を見る
In order to improve reproducibility of a quantitative hemagglutination inhibition for Human Chorionic Gonadotropin, effects of bovine serum albumin, normal rabbit serum and nonpregnant urine on the sensitivity of this test have been studied. As concentration of BSA in a diluent increases up to 1%, the sensitivity increases proportionally. Effect of 2% NRS is comparable to that of 0.1% BSA. Effect of nonpregnant urine on the sensitivity of the test is varied from one individual to another. mean effect of urine, however, nearly equals that of 0.1% BSA. From the comparison of a direct and a serial dilution method, and of dilution in cool and at temperature, it is suggested that the sensitivity increasing effect of protein could ascribed to its protective action to HCG molecules. The use of protein solution to dissolve standard HCG as well as urine samples is recommended for a quantitative or semi-quantitative assay of HCG in urine.
- 社団法人 日本内分泌学会の論文
著者
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Tamada Taro
Department Of Community And Family Medicine Jichi Medical School
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Matsumoto Seiichi
Department Of Orthopedic Oncology Cancer Institute Hospital Japanese Foundation For Cancer Research
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OGAWA Nobuhisa
Research Laboratory, Mochida Pharmaceutical Co., Ltd.
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TSUKUI YOSHIJI
Department of Obstetrics and Gynecology Gunma University School of Medicine
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TAMADA TARO
Department of Obstetrics and Gynecology Gunma University School of Medicine
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