The Fate of ACTH Released from Rat Anterior Pituitary into the Incubation Medium in vitro: Enzymatic Degradation and Acid Activation

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The bioactivity of ACTH released from isolated rat anterior pituitary glands into the incubation medium was determined.<BR>After the pituitaries were removed, ACTH activity in the medium decreased exponentially during further incubation at 37°C. The loss of ACTH activity was temperature-and pH-dependent and inhibited both by protease inhibitor (trasylol) and by preheating. Crude tissue extracts from median eminence, cerebral cortex and liver similarly inhibited the loss of ACTH activity.<BR>These results indicate that ACTH released into the medium may be destroyed by proteolytic enzyme (s) from the rat anterior pituitary.<BR>ACTH activity in the incubation medium was increased promptly by acidification of the medium to pH 1.5-2.5 with HC1, and reduced to the initial level by NaOH reneutralization of the medium (pH 6.8-7.8).<BR>These phenomena were not observed after the incubation medium had been heated at 100°C for 5 min.
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