Studies on <I>in Vitro</I> Synthesis and Secretion of Human Chorionic Gonadotropin and Its Subunits
スポンサーリンク
概要
- 論文の詳細を見る
Human chorionic tissues were cultivated <I>in vitro</I> in the presence of <SUP>3</SUP>H-proline in order to study the synthesis of hCG and its subunits by the placenta. After terminating the culture, tissue extracts and media were individually gel-filtrated on Sephadex G-100. The eluted fractions were radioimmunoassayed for hCG, hCGα and hCGβ and were measured for <SUP>3</SUP>H-radioactivity. Label incorporation was determined by immunoprecipitation. Elution profiles of tissue extracts showed the existence of large immunologic forms of hCG, hCGα and hCGβ emerging near the void volume. The amounts of these large immunologic species in chorionic tissue gradually decreased during the course of cultivation. <SUP>3</SUP>H-proline was almost exclusively incorporated into the large immunologic forms of hCG, hCGα and hCGβ within the chorionic tissue during the 5-hour exposure. A great quantity of hCGα was found in the media after the 3-day culture, while the amount of hCGβ found in the media was minute. After a 15-minute pulse, the <SUP>3</SUP>H-radioactivity peak within the chorionic tissue appeared in tha void volume, coincidental with hCG immunoreactivity. During the chase period, there was a shift of the <SUP>3</SUP>H-radioactivity peak associated with hCG immunoreactivity from the void volume to the more retarded area. In the media until after the 60-minute chase, no labeled hCG and its subunits appeared. Within the media after the 3-hour chase, the hCG peak associated with <SUP>3</SUP>H-radioactivity was more retarded on Sephadex G-100 than that within the tissue extract after the 15-minute pulse. These results suggest that the large immunologic forms of hCG, hCGα and hCGβ are synthesized as the earliest detectable biosynthetic forms and that they may then be converted to small molecule species.<BR>In the culture of molar trophoblastic tissues after a 15-minute pulse, considerable amounts of hCG and its subunits accompanied by high <SUP>3</SUP>H-radioactivity had already been secreted into the media. These observations suggest that protein synthesis by molar trophoblastic tissue is markedly enhanced as compared with that by normal chorionic tissue and that immunoreactive materials synthesized in molar trophoblastic tissue may be secreted more readily than those synthesized in normal chorionic tissue.
- 社団法人 日本内分泌学会の論文
著者
関連論文
- PJ-762 The Ratio and Lesion Morphology of Recurrent Restenosis after Reimplantation with Sirolimus-eluting Stent for Restenosis after Sirolimus-eluting Stent Implantation(PJ128,Coronary Revascularization, PCI (DES) 5 (IHD),Poster Session (Japanese),The 73
- PJ-478 The Detection of Cancer with Multidetecter Computed Tomography Coronary Angiography(PJ081,CT/MRI (Coronary/Vascular) 3 (I),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- PJ-408 Sirolimus-eluting Stent Implantation for In-stent Restenosis of Bare Metal Stent or Sirolimus-eluting Stent(PJ069,Coronary Revascularization, PCI (Restenosis/Others) 1 (IHD),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japane
- PE-379 Late Restenosis of Sirolimus Eluting Stent Implantation in Chronic Total Occlusions(PE064,Coronary Revascularization, PCI (DES) 6 (IHD),Poster Session (English),The 73rd Annual Scientific Meeting of the Japanese Circulation Society)
- PE-280 Unique Distribution of Stent Fracture and Stent Fracture with Restenosis after Sirolimus-Eluting Stent Implantation(PE047,Coronary Revascularization, PCI (DES) 4 (IHD),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circ
- PE-134 Is Previous Angiography as Follow up Useful to Predict Very Late Stent Thrombosis?(PE023,Coronary Revascularization, PCI (DES) 3 (IHD),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- OJ-255 Usefulness of Stress Myocardial Perfusion Imaging (MPI) in Detecting Significant Coronary Artery Stenosis in Multidetector Row Computed Tomography (MDCT) Era(OJ43,Nuclear Cardiology (Coronary, Myocardium) (I),Oral Presentation (Japanese),The 73rd A
- OJ-121 Predictors of Recurrent Restenosis after Treatment of Bifurcation Lesions with the Two-stent Strategy(OJ21,Coronary Revascularization, PCI (Complex Lesions) 2 (IHD),Oral Presentation (Japanese),The 73rd Annual Scientific Meeting of The Japanese Cir
- OE-403 Stent Fracture and Stent Thrombosis after Sirolimus-eluting Stent Implantation(OE68,Coronary Revascularization, PCI (Restenosis/Others) 4 (IHD),Oral Presentation (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- OE-188 Prevalence and Predictors of Early and Late Restenosis after Sirolimus-Eluting Stent Implantation for de novo Lesion(OE32,Coronary Revascularization, PCI (Restenosis/Others) 2 (IHD),Oral Presentation (English),The 73rd Annual Scientific Meeting of
- PJ-657 Optimal Strategy of Restenosis with Stent Fracture after Sirolimus-eluting stent Implantation(Coronary revascularization, PCI(24)(IHD),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-258 The 2-year Follow-up Results of Drug Eluting Stent Implantation for the de novo Unprotected Left Main Coronary Artery Disease.(Coronary revascularization, PCI(19)(IHD),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circ
- PE-049 The Long-Term Predictors of Restenosis and Target-Lesion-Revascularization after Drug-Eluting-Stent Implantation for the de novo Unprotected Left Main Coronary Artery Disease.(Coronary revascularization, PCI(07)(IHD),Poster Session(English),The 72n
- OJ-221 Late Luminal Response after Stenting in Chronic Total Occlusions : A Comparison between Bare Metal Stent and Sirolimus-Eluting Stent Implantation(Restenosis basic / clinical(02)(IHD),Oral Presentation (Japanese),The 72nd Annual Scientific Meeting o
- OE-004 Late Restenosis After Sirolimus-eluting Stent Implantation : The Impact of Lesion Complexity(Coronary revascularization, PCI(01)(IHD),Oral Presentation(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- FRS-025 Middle-term angiographic follow-up of BioMatrix^ stent implantation for left main to left anterior descending artery(Forefront Updates in PCI(01)(IHD),Featured Research Session,The 72nd Annual Scientific Meeting of the Japanese Circulation Soc
- P1-IS-85 Selective progesterone receptor modulator asoprisnil induces endoplasmic reticulum stress in cultured human uterine leiomyoma cells
- Effects of progesterone receptor modulator CDB2914 on apoptosis of cultured uterine leiomyoma cells(Oncology 12)
- 7-25.Effects of Selective Progesterone Receptor Modulator(SPRM) VA2914 on the Growth and Apoptosis of Cultured Uterine Leiomyoma Cells(Session 9 Others 1)
- 7-24.Direct Effects of GnRH Antagonist (Cetrorelix) on Proliferative Activity, Apoptosis and EGF Expression in Cultured Human Uterine Leiomyoma Cells(Session 9 Others 1)
- 6-24.Tumor Necrosis Factor α-1031T/C promoter polymorphism is associated with an increased risk of endometriosis in a Japanese population.(Session 7 Reproduction 1)
- 6-23.Polymorphisms in Estrogen Receptor β Gene but not Estrogen Receptor α Gene Affect the Risk of Developing Endometriosis in a Japanese Population(Session 7 Reproduction 1)
- IS-68 No Association of GSTM1 Null Mutation and NAT2 Gene Polymorphism with Endometriosis in a Japanese Population
- Myocardial Strain as a Potential Tool for Assessing Wall Function Quantitatively
- Impaired Myocardial Function in Aortic Stenosis is Improved Mainly in the Endocardial Side After Aortic Valve Replacement(Echo/Doppler 9 (I), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Myocardial Diastolic Dysfunction in Aortic Stenosis Assessed by a New Method Using Transmural Myocardial Strain Profile(Echo/Doppler 5 (I), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Screening of BRCA1 Mutation Using Immunohistochemical Staining with C-terminal and N-Terminal Antibodies in Familial Ovarian Cancers
- Large Calcified Mass on the Mitral Annulus
- PJ-334 Elevation of B-type Natriuretic Peptide and Right-to-Left Ventricular Geometric Changes after Transcatheter Closure of Atrial Septal Defect(Congenital heart disease/Kawasaki's disease-2, The 71st Annual Scientific Meeting of the Japanese Circulatio
- 4-10.Gleevec Sensitizes Ovarian Cancer Cells to CDDP(Session 5 Oncology 5)
- 1-17.Immunohistochemical demonstration of elevated expression of Laminin-5γ2 chain and MMP-2 in the neoplastic changes of cervical squamous epithelium.(Session 1 Oncology 1)
- Paradoxically abundant expression of Bcl-2 and adrenomedullin in invasive cervical squamous carcinoma
- Implications of adrenomedullin expression in the invasion of squamous cell carcinoma of the uterine cervix
- Transmural Myocardial Postsystolic Thickening and Dyssynchrony in Myocardial Infarction Assessed by Strain M-mode Imaging(New Ultrasound Technique (I), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- OJ-427 A Novel Method to Visualize Cardiac Electrical Current Using Magnetocardiogram : Application to Patients With Dilated Cardiomyopathy(ECG/Body Surface Potential Mapping/Holter 6 (A) : OJ52)(Oral Presentation (Japanese))
- FRS-001 Transmural Distribution of Myocardial Viability in Subendocardial and Transmural Infarction Assessed by Strain M-mode Imaging with Dobutamine Stress Echocardiography(Ultrasound Medicine in Cardiology (I) : FRS1)(Featured Research Session (English)
- Lazy Heart Muscle Demonstrated by Myocardial Strain Imaging
- Left Ventricular Systolic Asynchrony in Dilated Cardiomyopathy Quantified by Color Tissue Velocity Imaging
- Expression of Fas/Fas-Ligand, Bcl-2 Protein and Apoptosis in Extravillous Trophoblast along Invasion to the Decidua in Human Term Placenta
- IS-63 Effects of L-triiodothyronine(T3)on Cell Survival and Fas/Fas ligand Expression in Cultured Early Placental Extravillous Trophoblasts(EVT)
- Changes in Proliferative Potential, Apoptosis and Bcl-2 Protein Expression in Cytotrophoblasts and Syncytiotrophoblast in Human Placenta over the Course of Pregnancy
- Immunological evidence for the expression of Na/K-ATPase in the porcine ovary during follicular growth and regression(Session18 Reproduction4)
- Transfection of antisense-CGβgene into choriocarcinoma suppressed the cell proliferation and induced apoptosis(Session15 Oncology7)
- IS-55 Na^+/K^+-ATPase Activity in Porcine Granulosa Cells is Closely Related to the Cell Proliferation and Apoptosis
- Implication of Maternal Nitrogen Balance in the Regulation of Circulating Levels of Insulin-Like Growth Factor-I in Human Pregnancy
- Comparison of Immunocytologic Localization of Insulin-Like Growth Factor Binding Protein-4 in Normal and Polycystic Ovary Syndrome Ovaries
- 7-26.Comparative Effects of Heparin Binding-EGF Like Growth Factor(HB-EGF) on the Growth of Uterine Leiomyoma Cells and Normal Myometrial Cells(Session 9 Others 1)
- Immunohistochemical Evidence for the Presence of Tumor Necrosis Factor-α in the Infant and Adult Human Ovary
- Antenatal Use of Ambroxol for the Prevention of Infant Respiratory Distress Syndrome
- Effects of levonorgestrel-releasing intrauterine system on the expression of vascular endothelial growth factor and adrenomedullin in the endometrium(Session9 Reproduction1)
- Fundamental approach for ensuring ITER safety
- IS-136 The Influence of Socioeconomic Background and Antenatal Care Program on Maternal Mortality in Surabaya, Indonesia
- IS-42 Expression of single chain luteinizing hormone analogue
- Serial Clinical and Angiographic Follow-Up After Phosphorylcholine-Coated Stent Implantation
- Multiple Inhibitory Effects of a Lutenizing Hormone-Releasing Hormone Agonist on hCG-dependent Steroidogenesis and on FSH-dependent Responses in Ovarian Cells in vivo and in vitro
- In vitro Biosynthesis of Human Chorionic Follicle Stimulating Hormone
- Chorionic Gonadotropin Synthesized in Cultivated Trophoblast
- Studies on in Vitro Synthesis and Secretion of Human Chorionic Gonadotropin and Its Subunits
- Large Immunologic Species of Human Chorionic Gonadotropin in Placental Extracts
- Some Biological Properties of Human Chorionic Follicle Stimulating Hormone
- Three-dimensional echocardiographic pictures of isolated double-orifice mitral valve
- Giant-Cell Myocarditis Complicated by Heparin-induced Thrombocytopenia