Production of Anti-Rat Islet Cell Surface Antibody in Guinea Pigs

スポンサーリンク

概要

• 論文の詳細を見る

• Anti-rat islet serum was prepared in guinea pigs by multiple subcutaneous inoculations of rat islets homogenates emulsified in complete Freunds adjuvant (CFA). The anti-rat islet serum was cytotoxic against rat spleen cells in the presence of complement and the nonspecific antibodies were absorbed with homogenates of rat livers and spleens. After absorption, the serum lost the cytotoxicity against the rat spleen cells yet showed specific cytotoxicity against the rat islet cells. The binding capacity of anti-rat islet antibody was determined by the indirect immunofluorescence test using FITC conjugated rabbit anti-guinea pig IgG serum.<BR>As the guinea pig anti-rat islet serum contained anti-insulin antibody, the role of this antibody in this cytotoxic activity and surface immunofluorescence was studied. However, the anti-insulin antibody used as the control showed neither cytotoxicity nor surface immunofluorescence. After neutralizing the anti-insulin antibody in the antiserum with insulin, the serum remained cytotoxic to the rat islet cells and a surface immunofluorescence appeared.<BR>These data show that specific anti-rat islet cell surface antibody can be produced in guinea pigs by multiple inoculations of rat islets homogenates with CFA.

• 社団法人 日本内分泌学会の論文

著者

• Ono Junko

  Department Of Laboratory Medicine Fukuoka University School Of Medicine

• NAKAMURA Minoru

  The First Department of Internal Medicine, Faculty of Medicine, Kyusyu University

• Sekiguchi Naotaka

  Third Department Of Internal Medicine Faculty Of Medicine Kyushu University

• Takaki Ryosaburo

  Department Of Internal Medicine Oita Medical University

• NAGAFUCHI SEIHO

  The First Department of Internal Medicine, Faculty of Medicine, Kyushu University

• OKEDA TOSHIMITSU

  The First Department of Internal Medicine, Faculty of Medicine, Kyushu University

• YAMAGUCHI KEISUKE

  The First Department of Internal Medicine, Faculty of Medicine, Kyushu University

• Nakamura Minoru

  The First Department Of Internal Medicine Faculty Of Medicine Kyusyu University

• Ono Junko

  Department Of Clinical Chemistry And Laboratory Medicine Faculty Of Medicine Fukuoka University

• ONO JUNKO

  Department of Medicine, Medical College of Oita

関連論文

• 1994〜1996年臨床材料分離菌10菌種に対する 各種抗菌薬の抗菌力の推移-カルバペネム系抗菌薬を中心に-
• Liver X receptor in cooperation with SREBP-1c is a major lipid synthesis regulator in nonalcoholic fatty liver disease
• Lemierre's Syndrome : Porphyromonas asaccharolytica as a Putative Pathogen
• Body surface area is an independent factor contributing to the effects of lamivudine treatment
• Primary hepatic lymphoma in a patient with Sjogren's syndrome
• ANTI-MONOCYTE CHEMOATTRACTANT PROTEIN-1 GENE THERAPY PREVENTS DIMETHYLNITROSAMINE-INDUCED HEPATIC FIBROSIS IN RATS
• PREVENTION OF HEPATIC FIBROSIS BY MODULATING RHO-ROCK SIGNALING PATHWAY
• Serum levels of HCV RNA and core protein before and after incubation at 37℃ for 24h
• Reversibility of Serum NH_3 Level in a Case of Sudden Onset and Rapidly Progressive Case of Type 2 Citrullinemia
• Transforming Growth Factor-α Induces the Differentiation of Sarcomatoid Cholangiocarcinoma Cells
• Genetic analysis of expression profile involved in retinoid metabolism in non-alcoholic fatty liver disease
• THE RELATIONSHIP BETWEEN SERUM LIPOPROTEIN(a) AND RESTENOSIS AFTER INITIAL ELECTIVE PERCUTANEOUS TRANSLUMINAL CORONARY ANGIOPLASTY
• SUPPRESSION OF VENTRICULAR PREMATURE CONTRACTIONS CONTINUES AFTER THE WASHOUT OF ANTIARRHYTHMIC DRUGS
• Suppression of insulitis and diabetes in B cell-deficient mice treated with streptozocin: B cells are essential for the TCR clonotype spreading of islet-infiltrating T cells
• Direct evidence for the contribution of B cells to the progression of insulitis and the development of diabetes in non-obese diabetic mice
• Amelioration of Hyperglycemia in Streptozotocin-Induced Diabetic Rats Receiving a Marginal Mass of Islet Grafts by Troglitazone, an Oral Antidiabetic Agent
• Genome-wide linkage analysis of type 2 diabetes mellitus reconfirms the susceptibility locus on 11p13-p12 in Japanese
• Expression profile of lipid metabolism-associated genes in hepatitis C virus-infected human liver
• Dermatomyositis Associated with Invasive Thymoma
• Establishment and structural analysis of human mAb to E2 component of the 2-oxoglutarate dehydrogenase complex generated from a patient with primary biliary cirrhosis
• Increased mRNA expression of a novel prostacyclin-stimulating factor in human colon cancer
• A Case of Mitochondrial Encephalomyopathy, Lactic Acidosis and Stroke-like Episodes Associated With Diabetes Mellitus and Hypothalamo-Pituitary Dysfunction
• Inhibitory Effect of the α_1-Antitrypsin Pittsburgh Type-Mutant (α_1-PI_) on Proinsulin Processing in the Regulated Secretory Pathway of the Pancreatic β-Cell Line MIN6
• 2-1). Antigen Peptide Analog : Induction of T cell Anergy in Primary Biliary Cirrhosis
• -322- EFFECTS OF Ca-ANTAGONISTS ON THE BETA RECEPTOR OF CULTURED CARDIAC MYOCYTES
• EFFECT OF TRIIODOTHYRONINE ON THE SPONTANEOUS BEATING AND β-ADRENOCEPTOR OF NEONATAL RAT MYOCYTES : Myocardial Biochemistry : PROCEEDINGS OF THE 50th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
• -1202-THE EFFECT OF MILRINONE ON THE HEMODYNAMICS IN PATIENTS WITH CONGESTIVE HEART FAILURE : THE 54th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
• The suppressive effects of dietary protein restriction on the progression of renal impairment in OLETF rats
• PROLONGATION OF ACTION POTENTIAL DURATION IN VENTRICULAR MUSCLES FROM CHRONIC DIABETIC RAT : Pathophysiology : 53 Annual Scientific Meeting, Japanese Circulation Society
• The Purification of a GroEL-Like Stress Protein from Aerobically Adapted Campylobacter jejuni
• HEMODYNAMIC EFFECTS OF MILRINONE IN PATIENTS WITH CONGESTIVE HEART FAILURE : Short-and Long-term Follow Up Studies
• Iron state in association with retinoid metabolism in non-alcoholic fatty liver disease
• Production of Anti-Rat Islet Cell Surface Antibody in Guinea Pigs
• A polymorphism in the integrin αV subunit gene affects the progression of primary biliary cirrhosis in Japanese patients
• Evaluation of risk factors for the development of cirrhosis in autoimmune hepatitis : Japanese NHO-AIH prospective study
• Alpha-fetoprotein above normal levels as a risk factor for the development of hepatocellular carcinoma in patients infected with hepatitis C virus
• Simple Method for the Collection of Pancreatic Islets by the Use of Ficoll-Conray Gradient
• Changes in the Hypothalamo-Pituitary-Adrenal Function of Rats Exposed to Forced Walking Stress
• Prevention of Early Islet Graft Loss in Association with Engraftment in the Liver of Mice by Targeting IL-6/IL-6 Receptor Signaling
• Genetic polymorphisms in CTLA4 and SLC4A2 are differentially associated with the pathogenesis of primary biliary cirrhosis in Japanese patients
• Criteria for Early Identification of Aceruloplasminemia
• A case of arrhythmogenic right ventricular dysplasia with prominent late potential.
• The complete cancellation of abnormal Q waves due to an old anteroseptal infarction following subsequent acute posterior myocardial infarction.
• Mexiletine and Disopyramide Suppress Ventricular Premature Contractions(VPC) Irrespective of the Relationship between the VPC and the Underlying Heart Rate.
• Chronic EB Virus Infection and Cytokine: IL-2, Interferon Gamma, and BCRF1 Gene Product (Viral IL-10).

もっと見る 閉じる

スポンサーリンク