INFLUENCES OF PROTEOLYTIC ENZYMES ON PAROTIN VI. ISOLATION AND PROPERTIES OF A BIOLOGICALLY ACTIVE PROTEIN “PAROTIN-T. A” FROM THE PAROTIN-DIGEST BY TRYPSIN : STUDIES ON THE PHYSIOLOGICAL CHEMISTRY OF THE SALIVARY GLANDS LV

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概要

• 論文の詳細を見る

• Parotin and S-parotin were adsorbed largely on the acid alumina and slightly on the neutral alumina. In contrast with parotin and S-parotin, trypsin was adsorbed on the neutral alumina and not completely adsorbed on the acid alumina. Chymotrypsin was also not adsorbed on the acid alumina as same as trypsin. Thus the acid alumina column chromatography was found to be useful for the purification of parotin and S-parotin.<BR>The pH 4.5-precipitate of parotin-digest by trypsin showed the adsorption affinity on alumina column similarly as parotin. Parotin-T. A, the biologically active fraction in the somewhat degradated pH 4.5-precipitate, was isolated in homogeneous state by the column chromatography on acid alumina. The aqueous solution of parotin-T. A showed the ultraviolet absorption maximum at 275.5 to 276mμ of wave length. From the ultracentrifugal measurements, it was estimated that the sedimentation coefficient to be 3.16×10<SUP>-13</SUP>, the diffusion coefficient to be 3.10×10<SUP>-7</SUP>, and the molecular weight to be 99, 000. The amino acid component was found to be the following 16 kinds; alanine, arginine, aspartic acid, cystine, glutamic acid, glycine, histidine, leucine (and/or isoleucine), lysine, phenylalanine, praline, serine, threonine, tryptophan, tyrosine and valine.<BR>The N-terminal amino acids were identified as aspartic acid and glycine.<BR>The author would like to express his deep gratitude to Prof. Y. Ito of this institute for his kind advice during the course of this work. Thanks are also due to Mr. Y. Kubota, Miss M. Takahashi, Mrs. F. Suzuki and Mrs. H. Ogawara for their kind cooperation and advice for this work.

• 社団法人 日本内分泌学会の論文

著者

• Shinoda Masato

  Department Of Biochemistry Faculty Of Pharmaceutical Sciences Hoshi University

• Shinoda M.

  Department Of Fixed Prosthodontics Okayama University Dental School

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