有明海のり漁場すら分離したあかぐされ病原菌Pythiumに関する研究-III : 有性生殖器官形成の環境および栄養条件

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Investigations were made on environmental and nutritional conditions for the production of oogonia and oospores in Pythium strains causing laver red rot in Ariake Sea Farm. Five test strains produced conidia more abundantly than sexual organs, and also produced considerable numbers of oogonia on corn meal 50% sea water agar. But, most of oogonia were abortive an did not develope to mature oospores. The optimal ranges for the production of oogonia and oospores, from the results of two representative strains, were 40-60% sea water and 15-20°C for both strains, and pH 60.-7.0 for strain S1 and pH 7.0-8.0 for strain S2. However, even under such optimal conditions, oospores were not produced abundantly and we found no suitable medium for oospore production among 10 test media. Supplementing vegetable oils such as the oil of corn, soy bean, rapeseed or linseed to glucose-nitrate media were significantly stimulated oospore production, but sterols and laver lipid had little or no effect on its production. In comparative culture tests, Pythium strains from Ariake Sea produced oogonia and oospores less markedly than strains of Pythium porphyrae from Miyagi Prefecure of Tohoku.
日本水産學會の論文