ウニ脂質中のエイコサジエン酸位置異性体の分離

概要

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A fatty acid which had been tentatively identified as an isomeric eicosadienoic acid in previous papers, was separated in considerably high purity from the lipid of sea urchin, Anthocidaris crassispina, by using a variety of analytical techniques. The mixed methyl esters of fatty acids from sea-urchin lipid were subjected to wintering, fractional vacuum distillation, and urea fractionation. A fraction rich in the isomer was thus obtained. The fraction was purified further by argentation column chromatography, TLC on silica gel impregnated with n-undecane, silicic acid column chromatography and silica gel TLC. Two isomers of comparatively high purity were obtained after the final process, their purities, expressed as percentages according to GLC peak areas, being 97. 8% and 98. 4%, respectively. In addition to the results of GLC analysis, both chain length analysis and hydro-gen uptake measurement carried out with one of the final products gave evidence confirming that the isomer is eicosadienoic acid itself.
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