シロサケ脳下垂体性腺刺激ホルモンの精製

概要

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Purification of chum salmon gonadotropin was attempted. The pituitary glands of chum salmon were extracted with 40% ethanol. Ethanol was added to the extract to give a final 85% solution. The resulting precipitate was collected by centrifugation and dried with acetone. The crude gonadotropin powder obtained was filtered through a Sephadex G-100 column using 0.1M NH4HCO3, pH 8.3, resulting in the appearance of 3 peaks. Gonadotropic activity as assayed by the spermiation test was recognized only in the second fraction (80%response). This gonadotropic fraction (CSG•G100) was lyophilized and chromatographed on a DEAE-cellulose column with a linear gradient. The active fraction (100% response) was eluted in 0.165M NaCl-0.001M Tris-HCl, pH 7.0. When eluted stepwise with 0.08, 0.16, 0.24M NaCl, the gonadotropin (CSG•DEAE) was eluted at 0.16M. The gonadotropin exhibited a single band on polyacrylamide gel disc electrophoresis at pH 9.4.
公益社団法人日本水産学会の論文