アニサキス幼虫の食品衛生学的研究-II : アニサキス第III期幼虫の遊離および結合アミノ酸組成について

概要

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Anisakiasis is said to be caused by ingestion of the excreta or body constituents of Anisakis larva. To study the chemical composition of these materials, the determination of amino acid composition was carried out. The worms werg collected from Alaska pollack caught in the Okhotsk Sea and in the Pacific Ocean. The worms were washed with physiological saline, then extracted with 70% ethanol. The residue was hydrolyzed with 6 N HC1 at 110°C for 20hrs. The determinative results with a liquid chromatography make clear the following points. Obvious differences were found between the two extractives from Okhotsk and Pacific worms as follows: in the former, large quantities of glutamic acid and alanine were found, while in the latter, the content of alanine was found to be highest. Approximately the same results were obtained from both hydrolysates, both of which contains 17 kinds of amino acids. No special ninhydrin positive substances were found. The worms collected from Alaska pollack contained 3 or 6 times the aspartic acid and glutamic acid content of worms from mackerel, but relatively lesser amounts of tyrosine, histidine and arginine.
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