STUDIES ON THE EXTRACTIVES OF "UNI":II. NUCLEOTIDES AND ORGANIC BASES

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Succeeding to the previous paper1), in which the amino acid composition was given, the present paper deals with nucleotides and organic bases in the extracts of Uni. Analyses were carried out with ion-exchange chromatography on the extracts of four kinds of sample. The total amount of nucleotides which were mainly consisted of cytidylic, adenylic and uridylic acids was rather small and the quantity of both inosinic and guanylic acids was insignificant. Some of the extracts contained a small quantity of adenosine tri and diphosphates. Nucleoside was absent in every sample. Adenine, guanine, hypoxanthine, uracil and cytosine were common, but thymine was found only in one of the samples. Among these bases, hypoxanthine was most abundant (Table 1). Organic bases which are precipitated by ammonium reineckate were very scanty and few, and only betaine of a small amount was general throughout the samples (Table 2). As shown in Fig. 1, up to 95-98% of the extractive nitrogen were explained by the analyses carried out so far.
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