Biochemical Studies on the Fish Blood-IX:On the Paper Electrophoretic Analysis of Serum Proteins

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(1) In the paper electrophoretic analysis of the serum proteins of fish, it was found to be very important to select the kind of buffer solution, its pH and ionic strength. (2) The existence of such characteristics of the serum protein composition as reported in the former paper was equally ascertained. Moreover in the serum of both shark and skate, the existence of the component invested with the less mobility than that of γ-globulin contained in the human serum was also ascertained. (3) As the result of applying paper electrophoresis both to the shark serum containing large amounts of non-protein nitrogen compounds and to its dialysed one, it was ascertained that, from the qualitative analytical point of view, even in the case of the original serum, the influences of the co-existing non protein substances might safely be put out of consideration. (4) TISELIUS method was found to be more effective for separating each component protein than the paper electrophoresis. And under the ordinary condition, paper electrophoresis gives a higher value, relative proportion of component, to the fraction with low mobility and a lower to the fraction with high mobility than those obtained by TISELIUS method.
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