STUDIES ON THE MANUFACTURE OF CHONDROITIN ?? SULFATE FROM WHALE NASAL CARTILAGE BY ALKALINE LIQUEFACTION METHOD-III:DEPROTEINIZATION METHOD

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In the previous paper1)2), the preparation method of crude sodium chondroitin sulfate available for a medicine or food additive from a whale nasal cartilage has been studied. In order to obtain the product of high purity for a medical purpose, it is necessary to remove as exhaustively as possible the protein and its hydrolysates that are contaminants in the crude product. The present paper deals with a trial to find out an effective and simple method of deproteinization. The cartilage was liquefied with 50% NaOH for 1.75-2.5 hrs at 40°C. After dialysis followed by deproteinization at pH 3.5, the liquefied solutions were deproteinized with active carbon, Japanese acid clay or ion exchange resins, and then sodium chondroitin sulfate was precipitated with ethanol. All these treatments were effective, and in particuler the product obtained by the treatment with Dowex 50 W-X 1 was negative in biuret reaction (Table 1, 2, 3). Moreover it was found that dialysis caused low purity in the product (Table 4). In the solution of crude sodium chondroitin sulfate obtained with omission of dialysis, these treatments gave rise to more effective results than in the liquefied solution which was dialyzed. Thus, all products were negative in biuret reaction, and the order of yield was as follows: Dowex 50 W-X 1>active carbon>Japanese acid clay (Table 5).
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