STUDIES ON THE MANUFACTURE OF CHONDROITIN SULFATE FROM WHALE NASAL CARTILAGE BY ALKALINE LIQUEFACTION METHOD-II:PREPARATION OF CRUDE CHONDROITIN SULFATE BY LIQUEFACTION AT ROOM TEMPERATURE
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In the previous paper1), the alkaline liquefaction of a whale nasal cartilage at above 30°C has been studied for the industrial manufacture of chondroitin sulfate. The best condition was to liquefy it with 50% NaOH at 40°C for 1.5 hours. The present paper deals with the alkaline liquefaction at room temperature. The cartilage boiled in water was steeped in NaOH solution to be liquefied. After dialysis followed by deproteinization at pH 3.5 and neutralization, crude chondroitin sulfate was precipitated with ethanol. The influence of alkaline liquefaction conditions on the purity of crude chondroitin sulfate and the amount of extracted sodium chondroitin sulfate obtained by multipling the yield by the purity was discussed and the following results were obtained. 1) The boiled cartilage could be easily liquefied with 5% NaOH in one day at 20±2°C, although the raw cartilage could not be liquefied even with 10% NaOH. 2) In liquefaction for one day, the higher the concentration of NaOH solution was, the higher the purity of the product was (Fig. 1). On the whole, the longer the liquefaction time was, the higher the purity was, but in case of liquefaction with 3% and 10% NaOH a lowering of the purity was observed at 3-6 day (Figs. 2, 3). 3) The decrease in the concentration of ethanol used for the precipitation of crude chondroitin sulfate resulted in a significant elevation of the purity, attending a slight decrease in the amount of extracted sodium chondroitin sulfate (Table 1). 4) It was recomended as a suitable liquefaction condition to liquefy 100g of boiled cartilage with 100ml of 10% NaOH at 20±2°C for 2 days. 5) As compared with the heating liquefaction method, the present method gave rise (to the product lower in the purity, while little difference was found among the methods in the recovery of hexosamine from the cartilage.
- 社団法人 日本水産学会の論文
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