Demonstration of the dosage effect of Rho(D) antigens on human red blood cells by an EA rosette assay.
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概要
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Utilizing an EA rosette assay, we were able to distinguish genotypic differences of the Rh<SUB>0</SUB> (D) antigen on human red blood cells (RBC) based on the number of rosettes formed between human peripheral B lymphocytes and RBC optimally sensitized with anti-Rh<SUB>0</SUB> (D) antibody. In this study, RBC with possible Rh genotypes R<SUB>1</SUB>R<SUB>1</SUB> (CDe/CDe), R<SUB>1</SUB>r (CDe/cde), R<SUB>2</SUB>R<SUB>2</SUB> (cDE/cDE), R<SUB>2</SUB>r (cDE/cde) and R<SUB>1</SUB>R<SUB>2</SUB> (cDe/cDE) were sensitized with either Rh<SUB>0</SUB>GAM (anti-D) or Ripley serum (anti-G). When RBC were optimally sensitized with Rh<SUB>0</SUB>GAM, R<SUB>1</SUB>r formed 60% as many rosettes as R<SUB>1</SUB>R<SUB>1</SUB> and R<SUB>2</SUB>r formed 64% as many rosettes as did R<SUB>2</SUB>R<SUB>2</SUB>. Using Ripley serum for sensitization and comparing the number of rosettes formed, R<SUB>1</SUB>r exhibited 70% of R<SUB>1</SUB>R<SUB>1</SUB> and R<SUB>2</SUB>r showed 76% R<SUB>2</SUB>R<SUB>2</SUB>. Furthermore, R<SUB>1</SUB>R<SUB>1</SUB> cells sensitized with Ripley serum formed a higher percentage of rosettes than R<SUB>1</SUB>R<SUB>2</SUB> or R<SUB>2</SUB>R<SUB>2</SUB>.
- 久留米大学医学部 The Kurume Medical Journal 編集部の論文
著者
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YOKOYAMA M.
Department of Anstomy, Shimane Medical University Department of Molecular Life Science II, School of
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HORITA MASAKAZU
Department of Pathology and Microbiology University of Illinois at the Medical Center Chicago
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JABS ARTHUR
Department of Pathology and Microbiology University of Illinois at the Medical Center Chicago
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RICKER BARBARA
Department of Pathology and Microbiology University of Illinois at the Medical Center Chicago
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LOREN ALAN
Department of Pathology and Microbiology University of Illinois at the Medical Center Chicago
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