Determination of homozygote vs heterogote of blood group antigens with protein A.
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概要
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By sensitizing human red blood cells with corresponding antisera specific to blood group antigens and subsequent rosette formation with Staphylococcus aureus, we were able to distinguish homozygosity of the antigens from heterozygosity. Three blood group systems : Rh, Kell and Kidd were used in this study. In the Rh blood group system, we were able to demonstrate the dosage effect of the Rh<SUB>0</SUB> (D) antigen by rosette assay in addition to determining that the deletion of the C and/or E alleles increased the rosette forming cell count when red blood cells with different genotypes were sensitized with anti-D. Our assay proved to be simple, specific, highly reproducible, and can be adapted to study virtually any antigenic markers on a cell surface.
- 久留米大学医学部 The Kurume Medical Journal 編集部の論文
著者
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YOKOYAMA M.
Department of Anstomy, Shimane Medical University Department of Molecular Life Science II, School of
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LOREN ALAN
Department of Pathology and Microbiology University of Illinois at the Medical Center Chicago
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JABS DEAN
Department of Pathology and Microbiology, University of Illinois College of Medicine
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CHARMAN DAVID
Department of Pathology and Microbiology, University of Illinois College of Medicine
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