Role of Heparan Sulfate Proteoglycan (Syndecan-1) on the Renal Epithelial Cells During Calcium Oxalate Monohydrate Crystal Attachment
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概要
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We have reported that heparan sulfate (HS)/heparan sulfate proteoglycan (HSPG, syndecan-1) expression significantly increased in the rat kidney during calcium oxalate (CaOx) nephrolithiasis. Although the exact role of syndecan still remains unclear, HS/syndecan-1 is thought to have some important role in the CaOx crystal formation. Mardin-Darby canine kidney (MDCK) cells are most commonly used in kidney stone research. It was reported that MDCK cells do not express syndecan-1. In the present study, we established a novel MDCK cell line (KIC-synd-1) that expressed the human syndecan-1 gene. In this cell line, we confirmed stable expression of both sndecan-1 gene and core protein. Immunohistochemical study revealed positive staining of syndecan-1 monoclonal antibody in the basolateral and cytosolic area of the KIC-synd-1 cells. We also investigated the composition of glycosaminoglycans (GAGs) side chains in MDCK cells and KICsynd-1 cells by using high-performance liquid chromatography (HPLC). Four types of HS chains were identified in both cells as follows; ΔdiHS-NS, ΔdiHS-6S, ΔdiHS-diS<SUB>1</SUB>, ΔdiHS-diS<SUB>2</SUB>. Increased production of ΔdiHS-NS and ΔdiHS-diS<SUB>2</SUB> were shown in KIC-synd-1 cells compared with production in MDCK cells (p<0.05). In contrast, only a small amount of ΔdiHS-6S and ΔdiHS-diS<SUB>1</SUB> was contained in both cell lines. Total amount of HS was significantly increased in the KIC-synd-1 cells compare with that in the wild type MDCK cells (p<0.05). Scanning electron microscopy revealed no significant difference between cell surface of wild type MDCK cells and that of KIC-synd-1 cells in normal conditions. However, calcium oxalate crystal attachment was apparently decreased in the KIC-synd-1 cells. These results suggested that cell surface HS/syndecan-1 has preventive role for calcium oxalate nephrolithiasis via creation of a charge barrier against COM crystal attachment.
著者
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Noda Shinshi
Department Of Urology
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Kawagoe Nobutoshi
Department Of Immunology Kurume University School Of Medicine
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Matsuoka Kei
Department Of Geosystem Engineering The University Of Tokyo
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Iida Shizuka
Department Of Urology Iida Clinic
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Inoue Michiro
Department Of Urology Kurume University School Of Medicine
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Tomiyasu Katsuro
Department Of Urology Kurume University School Of Medicine
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MATSUOKA KEI
Departments of Urology, Kurume University School of Medicine
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KAWAGOE NOBUTOSHI
Departments of Urology, Kurume University School of Medicine
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CHIKAMA SHUMEI
Departments of Urology, Kurume University School of Medicine
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TAKAZONO ISOKO
Departments of Chemistry, Kurume University School of Medicine
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TAKAZONO ISOKO
Department of Chemistry, Kurume University School of Medicine
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